missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva HisTrap HP, 100 Columns

Cytiva HisTrap HP, 100 Columns

Product Code. 11880073 Shop All Cytiva Products
Change view
Click to view available options
Quantity:
100 x 1 mL
100 x 5 mL
Bed Volume:
1 mL
5 mL
Unit Size:
Pack of 100
Product Code. Quantity Bed Volume unitSize
11880073 100 x 5 mL 5 mL Pack of 100
11555674 100 x 1 mL 1 mL Pack of 100
2 options
This item is not returnable. View return policy

Product Code. 11880073

Brand: Cytiva 17524805

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

Ensure reliable, high-yield capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap HP, 100 Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification by immobilized metal ion affinity chromatography (IMAC).

HisTrap HP 1ml and 5ml columns are designed for one-step purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose High Performance to ensure reliable capture of target protein in repeated IMAC purifications. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield. At the same time, it greatly expands the range of suitable operating conditions.
  • High binding capacity—at least 40mg/ml chromatography medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump or chromatography system, such as ÄKTA design
  • Can be used for purification of a range of tagged proteins
  • 100-column bulk pack for convenience and economy
HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be co-purified with the tagged target protein.For convenient scaling up of histidine-tagged protein purification, use 20 ml HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.
TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 5 mL
Binding Capacity At least 40 mg histidine-tagged protein/mL medium, Protein binding capacity is protein-to-protein dependent.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene (PP)
Matrix Highly cross-linked agarose, 6%
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 34 μm
Quantity 100 x 5 mL
Type Pre-packed Column

Safety and Handling

Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.