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Cytiva HisTrap™ FF Columns: 7 x 25mm

Product Code. 11890073 Shop All Cytiva Products
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100 x 5 mL
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Pack of 100
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11890073 100 x 5 mL Pack of 100
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Product Code. 11890073 Supplier Cytiva Supplier No. 17525502

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Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cells

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 7 × 25mm
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity
Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12
TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 5 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Flow Rate <4 mL/min.
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C, 20% Ethanol
Max. Operating Pressure 5 bar
Quantity 100 x 5 mL
Type Pre-packed Column
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