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Description
Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins. Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis.
• Optimized—removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
• Fast—process samples in ∼10 minutes
• Economical—cost-effective spin columns are priced for single use
• Reduced carry-over—one-time use prevents protein carryover and experimental variability
High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.
Specifications
Specifications
| For Use With (Equipment) | Mass Spectormeter |
| Product Type | Mini Spin Column |
| Product Line | High Select |
| Content And Storage | Store at 2–8°C. |
| Label or Dye | Unlabeled |
| Shipping Condition | Wet Ice |
| Starting Material | Plasma, Serum |
| Final Product Type | Protein |
| Format | Mini Spin Column |
| Workflow Step | Protein Depletion |
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Frequently Asked Questions (FAQs)
No, the High Select HSA/Immunoglobulin Depletion Resin cannot be used for mouse samples; the depletion resin contains immobilized antibodies that only recognize human serum or plasma proteins.
We don’t recommend reusing the High Select HSA/Immunoglobulin Depletion Resin as depletion efficiency may decrease after regeneration, and subsequent depleted samples may be contaminated from prior ones.
The High Select HSA/Immunoglobulin Depletion Resin is offered in 3 formats:
a) Resin alone: High Select HSA/Immunoglobulin Depletion Resin (Cat. No. A36368)
b) High Select HSA/Immunoglobulin Depletion Mini Spin Columns (Cat. Nos. A36365, A36366) that can process a maximum of 10 µL of human serum or plasma
c) High Select HSA/Immunoglobulin Depletion Midi Spin Columns (Cat. No. A36367) that can process a maximum of 100 µL of human serum or plasma. For serum samples containing abnormally high amounts of albumin or IgG, the sample load may need to be reduced.
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