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Thermo Scientific™ pUC57 DNA

Product Code. 10765071 Shop All Thermo Scientific Products
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Quantity:
50 μg
Unit Size:
50µg
Product Code. Quantity unitSize
10765071 50 μg 50µg
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Product Code. 10765071

Brand: Thermo Scientific™ SD0171

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This item is not returnable. View return policy

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3'-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (±1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.

Highlights

  • More than 90% in the supercoiled form
  • Isolated from E. coli (dam+, dcm+)
  • Purified by chromatography using proprietary patented technology
TRUSTED_SUSTAINABILITY

Specifications

Cloning Method Restriction Enzyme⁄ MCS
Concentration 0.5 μg/μL
Sample Type DNA
Final Product Type DNA
For Use With (Application) Cloning
Product Type DNA
Quantity 50 μg
Vector pUC57

For Research Use Only. Not for use in diagnostic procedures.

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