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  5. Invitrogen™ Human D-Dimer ELISA Kit

Invitrogen™ Human D-Dimer ELISA Kit

Produktkod. 17155419
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Förpackningsstorlek:
1 set
Each
Pack of 10
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Produktkod. 17155419

Brand: Invitrogen™ EHDDIMERX5

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Beskrivning

ELISA

The Human D-Dimer ELISA quantitates Hu D-Dimer in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu D-Dimer. Principle of the method The Human D-Dimer solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.
TRUSTED_SUSTAINABILITY

Specifikationer

Accession Number P02671
Assay Range 0.082 to 60 pg/mL
Assay Sensitivity 0.08 pg/mL
Calibration Range 0.08 pg/mL to 60 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cell Structure & Organelle Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human D-Dimer. Other species not determined.
Sufficient For 5 x 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2243
Gene Symbol Fib2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Extracellular Matrix & Cell Adhesion
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym Fib2
Target D-dimer
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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