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Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry
Beskrivning
Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.
Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.
Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.
To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.
Specifikationer
Specifikationer
| Quantity | 0.5 mg |
| Kit Contents | Contains 0.5 mg of Alexa Fluor 488 sDIBO Alkyne |
| Molecular Weight (g/mol) | ∼1400 |
| Filter Type | GFP |
| Content And Storage | Store at ≤-20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time |
| Flow Cytometer Laser Lines | Blue |
| Labeling Method | Click Chemistry |
| Shipping Condition | Dry Ice |
| Reactive Moiety | sDIBO Alkyne |
| Label or Dye | Alexa Fluor™ 488 |
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Frequently Asked Questions (FAQs)
For Click-iT sDIBO Alkynes for coper-free click chemistry (Cat. Nos. C10407, C20020, C20021, C20022, C20023, C20025), we recommend a 2-5 mM stock solution in DMSO.
Structurally, they are the same. The only difference is in the manner of packaging. SiteClick Alexa Fluor 488 sDIBO Alkyne is packaged for labeling 1 to 5 mg of antibody per the SiteClick labeling protocol with the actual amount being proprietary. Click-iT Alexa Fluor 488 sDIBO Alkyne is packaged as a 0.5 mg of reagent for use with any copper-free click reaction.
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