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  5. Cytiva Cell Proliferation Kit, BioTrak™ ELISA Kit and Components

Cytiva Cell Proliferation Kit, BioTrak™ ELISA Kit and Components

Product Code. 10004694 Shop All Cytiva Products
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Quantity:
130 μL
10 mL
Unit Size:
10mL
4mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Includes

The cell proliferation kit includes:
Labeling reagent, Nuclease, Anti-5-bromo-2'-deoxyuridine monoclonal antibody, Peroxidase anti-mouse IgG2a, Peroxidase anti-mouse IgG2a diluent, Substrate/intensifier

Cel proliferation Biotrak ELISA kit includes:
BrdU labelling reagent; Fixative; Blocking reagent; Peroxidase-labelled anti-BrdU; Antibody dilution solution; Wash buffer; Substrate solution

Cell proliferation labelling reagent and Anti-BrdU antibody are available separately

Provides a precise, fast and simple colorimetric assay to quantitate proliferating cells cultured in a 96 well microplate

  • Recommended cell number: 10e6-10e7 cells/mL (Cell proliferation kit) 10e2 cells/well-10e5 cells/well (Biotrak ELISA kit)
  • Required time: 2-3 hour post-labeling (labeling 1-2 hours)
  • Antibody and labeling reagent available separately for ELISA and ICC systems
  • Suitable for in vivo and in vitro tissue labeling in addition to cultured cells (ELISA format)
  • Non radioactive protocols provide sensitivity comparable to autoradiographic methods
  • Cell proliferation kit avoids the need to chemically denature DNA. Cellular morphology is preserved allowing multiple labeling experiments
  • 5-fluoro-2'-deoxyuridine is used to inhibit thymidilate synthetase increasing BrdU incorporation

When ordering the Cell Proliferation Kit (RPN20), you must also order DAB REAGENT OF RPN20/RPN1174 (RPNK8888)

TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Includes Anti-5-2'-deoxyuridine Monoclonal Antibody Nuclease
Quantity 130 μL
Content And Storage 2°C to 8°C
For Use With (Application) Immunocytochemistry

Safety and Handling

compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : deoxyribotrometamolnuclease
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