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IBA Lifesciences CD9 Fab-TACS™ Exosome Agarose Column Starter Kit

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17207750 Human
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Code produit. 17207750 Fournisseur IBA Lifesciences Code fournisseur 63319002

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Description

Label-free isolation of CD9+ exosomes from cell culture media, serum or plasma

  • Fab-TACS™ is a traceless affinity exosome isolation approach based on our Strep-tag™ technology
  • CD9 Fab-TACS™ Exosome Agarose Column Starter Kit allows label-free isolation of human CD9+ exosomes from plasma, serum and cell culture supernatants
  • CD9 is a member of the tetraspanin family present on the surface of exosomes
  • Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix
  • Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target particles are efficiently washed away
  • In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the exosome surface due to their low affinity
  • The label-free, fully functional exosomes are now ready for further downstream applications.

Spécification

Includes Strep-Tactin™ TACS Agarose columns, 0.3 mL (2 x), CD9 Fab-Strep (human), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter
Target Species Human

For Research Use Only.

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