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CD16 Monoclonal Antibody (3G8), NovaFluor™ Blue 555, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1756.00 NOK - 3895.00 NOK
Specifications
Antigen | CD16 |
---|---|
Clone | 3G8 |
Concentration | 4 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
17216516
|
Invitrogen
H006T02B03 |
25 Tests |
1756.00 NOK
Pack of 25 |
Estimated Shipment: 10-05-2024 Log in to see stock. |
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17266526
|
Invitrogen
H006T03B03 |
100 Tests |
3895.00 NOK
Pack of 100 |
Estimated Shipment: 10-05-2024 Log in to see stock. |
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Description
This 3G8 monoclonal ab reacts with human and Non-human Primate CD16,also known aslow-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in Non-human Primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in ab-dependent cell-mediated cytotoxicity(ADCC), phagocytosis, cytokine release, and antigen presentation.
Based on cross-blocking studies 3G8 recognizes the same epitope as CB16. However, 3G8 and B73.1 ab clones bind distinct epitopes.
Applications Reported: This 3G8 ab has been reported for use in flow cytometric analysis.
Applications Tested: This 3G8 ab has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 4uL(0.6ug) per test. A test is defined as the amount(ug) of ab that will stain a cell sample in a final volume of 100uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 496 nm; Emission: 555 nm; Laser: 488 nm(Blue) Laser
Specifications
CD16 | |
4 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16B; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); | |
IgG1 | |
Affinity chromatography | |
Antibody |
3G8 | |
Flow Cytometry | |
NovaFluor Blue 555 | |
Mouse | |
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Rhesus Monkey | |
A3RFZ7, O75015, P08637 | |
2214, 2215, 720006 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
FCGR3A |