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Invitrogen™ IL-4 Bovine Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ ESS0031

3486.84 NOK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.10000kg

Product Code. 10177433

  • 5230.00 NOK / 1 set
Estimated Shipment: 11-04-2024
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Description

Description

The Bovine IL-4 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-4. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
Specifications

Specifications

P30367
<15.6 pg/mL
Biotin
ELISA Kit
No significant cross-reaction with recombinant bovine IL-1ß, IL-2, IL-6, IL-8, IFNg or TNFa; no significant cross-reaction with recombinant human, equine, mouse, porcine, rat, or rhesus macaque IL-4
Bovine
280824
Coating Antibody, Lyophilized Recombinant Standard, Detection Antibody, Streptavidin-HRP, Substrate Solution, Ready to use Stop Solution, 0.16M Sulfuric Acid, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Absorbance at 450nm minus absorbance at 550nm
RUO
2°C to 8°C
24 hr.
15.6-1000 pg/mL
Bovine IL-4 ELISA Reagent Kit
Colorimetric ELISA (TMB substrate) with Plates
Serum, Supernatant
5 x 96 microplate wells
Colorimetric Microplate Reader
BSF-1,IL-4
HRP
5 x 96 Tests
Serum, 100 μL; Supernatant, 100 μL
Bovine
3 hr. 20 min.
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