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  5. Invitrogen™ Mouse ANGPTL3 ELISA Kit

Invitrogen™ Mouse ANGPTL3 ELISA Kit

Product Code. 17175679
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 17175679

Brand: Invitrogen™ EMANGPTL3X5

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Description

ELISA

The Mouse ANGPTL3 ELISA quantitates Ms ANGPTL3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ANGPTL3. Principle of the method The Mouse ANGPLT3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9R182
Assay Range 40.96 to 10,000 pg/mL
Assay Sensitivity 40 pg/mL
Calibration Range 40 pg/mL to 10,000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Proteases, Peptidases & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6 Ckine, Activin A, ADAMTS1, Adiponectin, Angiopoietin-3, ANGTL-4 Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin, IFN-R1, IL-17 B, IL-17 B R, IL-22, MIP-1 b, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGFb1, TNF RI, TNF RII, TPO, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2, VCAM-1, VEGF.
Sufficient For 5 x 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 30924
Gene Symbol hypl
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Endocrinology
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP23-101O16.2, hypl
Target ANGPTL3
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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