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  5. Invitrogen™ Human Angiopoietin 1 ELISA Kit

Invitrogen™ Human Angiopoietin 1 ELISA Kit

Product Code. 15484893
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
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1 set
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Pack of 10
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15484893 96 Tests 1 set
17804180 10 x 96 Tests Pack of 10
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Product Code. 15484893 Supplier Invitrogen™ Supplier No. EHANGPT1

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Description

ELISA

The Human Angiopoietin 1 (Hu APO1) ELISA quantitates Hu APO1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO1. Principle of the method The Human APO1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q15389
Assay Range 74.07 to 18,000 pg/mL
Assay Sensitivity 30 pg/mL
Calibration Range 30 pg/mL to 18000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Kinase & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 284
Gene Symbol AGP1,AGPT,ANG1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Angiogenesis
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym AGP1, AGPT, ANG1
Target Angiopoietin 1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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