Invitrogen™ Aluora™ Spatial Amplification Reagent Components

Revolutionary signal amplification technology

The Aluora spatial amplification kits represent a breakthrough in signal amplification for spatial proteomics. They employ proprietary Aluora reagents that react with horseradish peroxidase (HRP) to deposit photostable Aluora dyes onto target proteins, significantly improving the signal-to-background ratio. This technology enables robust multiplexed protein detection even in challenging tissue samples. Aluora kits require 10–5000X less primary antibody than standard IHC to achieve equivalent signal intensity.

Aluora spatial amplification reagents are optimized for use with Prolong Glass Antifade mountants and 1 mg/mL Thermo Scientific DAPI nucleic acid stains for multiplex analysis.

Aluora reagents and dyes can be purchased individually or in pre-configured kits to detect mouse, rabbit, or biotin-labelled antibodies. The Rainbow kit is configured with all Aluora colors with 4X GAM and 4X GAR secondary detection core reagents for 100 slides in 8-plex.

Features of Aluora spatial amplification reagents include:

• Signal amplification for enhanced detection sensitivity
• Comprehensive spatial analysis and imaging capabilities for research
• Fluorescent detection system for multiplexing and quantitative analysis
• Flexible workflow options that accommodate diverse experimental needs
• Polymerase HRP (PolyHRP) conjugated secondary antibodies that boost signal for low abundance targets
• Highly cross-adsorbed secondary antibodies that help ensure target-specific detection and background reduction
• Enhanced background reduction through proprietary blocking reagents for crisp, clear imaging

Optimized for automated slide staining

A major benefit of Aluora spatial amplification kits is their compatibility with automated slide stainers right out of the box. This enables complete labeling of a tissue slide with an 8-plex Aluora panel in just 12–16 hours.

Eliminating background for cleaner images

Aluora spatial amplification kits include blockers to help reduce endogenous peroxidase activity and autofluorescence. By minimizing background noise, less primary antibody is needed to generate a strong specific signal.

Iterative labeling enables unlimited multiplexing

The covalently bound Aluora dyes are resistant to antibody stripping, remaining brightly fluorescent through subsequent labeling cycles. This enables iterative staining of numerous targets using primary antibodies from the same host species—a major constraint in traditional immunohistochemistry. Any number of directly conjugated primary antibodies can also be introduced after Aluora labeling is complete.

Custom optimization for diverse applications

Although general protocols are provided, each sample requires optimization for optimal results. Tissue preparation and processing methods vary widely and can profoundly impact staining. Careful optimization of the complete workflow is critical to achieve the highest quality multiplexed data from precious samples. Aluora spatial amplification provides a versatile tool to address diverse spatial proteomics applications.