Molecular Biology Methods
1. Isolation and Purification
Well-prepared nucleic acids are critical to your experiments and research, so how you isolate and purify your samples is important. Multiple methods exist for disrupting the cells, removing debris, capturing your sample, removing contaminants, and eluting the sample for use in downstream applications. Find products for isolation, extraction, purification, and more to complete this step of your process.
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Catalog Number 16416159
Cytiva Sera-Xtracta Cell-Free DNA Kit
Catalog Number 12703057
MP Biomedicals™ FastRNA™ Pro Soil-Direct Kit
2. Labeling and Detection
Tags or labels, called probes, are added to nucleic acids to enable detection or purification. Labels may include radioactive phosphates, biotin, fluorophores, and enzymes. Fluorescent in situ hybridization (FISH) is used to identify specific DNA sequences and tests can also be performed for pathogen detection. Find labeling and detection kits, spectrophotometers, fluorometers, and more below for your nucleic acid work.
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Catalog Number 7235448
Invitrogen™ Qubit™ Flex Starter Packages
Catalog Number 6909043
Thermo Scientific™ GENESYS™ 30 Visible Spectrophotometer
Catalog Number 3676034
Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System
3. Amplification
The goal of PCR is the amplification of existing DNA. Ultimately, having multiple copies makes it possible to perform your assays. Choose from a variety of thermal cyclers, polymerases, master mixes, and other PCR systems to support your PCR amplification needs.
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Catalog Number 15304502
Applied Biosystems™ ProFlex™ PCR System, 2 x 384-well
Catalog Number 7184433
Thermo Scientific™ dNTP Mix (25 mM each)
4. Cloning and Modification
Cloning and genetic modification can be used to research and identify genetic defects. Cell and gene therapy are expanding fields of interest. Choose from a variety of competent cells, PCR cloning kits, enzymes and inhibitors, and more.
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Catalog Number 4521711
Thermo Scientific™ FastDigest EcoRI
Catalog Number 16575231
Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells
Catalog Number 15930583
Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells
5. Transfection
Mimicking natural viral processes, transfection artificially introduces nucleic acids to cells, producing various chemical, biological, or physical changes. Although the term transfection was originally used to describe the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, the term currently refers to any artificial introduction of foreign nucleic acid into a cell. Search for transfection reagents, electroporation supplies, and more to help effectively conduct your transfection procedures.
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Catalog Number 15898441
BTX™ ECM™ 630 Exponential Decay Wave Electroporation System Complete, with Safety Stand
Catalog Number 10431915
Invitrogen™ Neon™ Transfection System
Catalog Number 6960076
Invitrogen™ Invivofectamine™ 3.0 Reagent
6. Analysis
The yield from your PCR and molecular biology procedures must be further analyzed and evaluated to confirm the outcome of your assays, especially if the material is intended for other downstream applications. Shop electrophoresis equipment, gel stains, imaging and documentation systems, and more to help ensure the success of your molecular biology assays.
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Catalog Number 16514341
Syngene™ Airclean 600 PCR Workstation
Catalog Number 15879922
Syngene™ G:BOX - F3 with Feedback Lens
Catalog Number 10328162