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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,643)
Magnetic Affinity Separation Particles (259)
Protease Inhibitors and Phosphatase Inhibitors (131)
Spin Columns and Gravity-flow Columns (119)
Gel Filtration Media and Columns (116)
Miscellaneous Immobilization Supports (110)
Cell Lysis Detergents (78)
In vitro Protein Expression Systems (41)
Affinity Gels (36)
Biotin-Binding Affinity Resins and Beads (34)
Miscellaneous Immobilized Affinity Ligands (29)
Cell Lysis Enzymes (17)

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1 – 15 of 1,625 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2
Promotions Available

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor
Form Liquid
Product Type Protease Inhibitor Cocktail
Reagent Type Protease Inhibitor
Product Line Halt™
3
Promotions Available

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce™
Detection Method Colorimetric
4
Promotions Available

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

EDGE
5

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
6

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

7

Gibco™ AAV-MAX Lysis Buffer

AAV-MAX Lysis Buffer is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells. Cell lysis is detergent-based, eliminating the need for mechanical cell disruption.

EDGE
8
Promotions Available

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor, Phosphatase Inhibitor
Form Liquid
Product Type Protease and Phosphatase Inhibitor Cocktail
Reagent Type Protease Inhibitor, Phosphatase Inhibitor
Product Line Halt™
9
Promotions Available

Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

EDGE
Content And Storage Upon receipt store product at 4°C. Do not freeze.
Inhibitors Phosphatase Inhibitor
Form Liquid
Product Type Phosphatase Inhibitor Cocktail
Reagent Type Phosphatase Inhibitor
Product Line Halt™
10

Proteintech ChromoTek GFP-Trap™ Magnetic Agarose

The ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins.

Target AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Content And Storage Upon receipt store at 4°C. Do not freeze.
Binding Capacity 10 μL slurry bind more than 8 μg of recombinant GFP
Label or Dye Magnetic Agarose
For Use With (Application) IP, CoIP, ChIP, RIP
Species Alpaca
11
Promotions Available

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

EDGE
Particle Size 1 μm
Ligand Type Antibody
Type Magnetic Affinity Bead
Target HA
Content And Storage Store at 4°C.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10mg/mL, 1% solids
Product Line Pierce™
12
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
dimMatrix Magnetic Agarose
13
Promotions Available

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal
14
Promotions Available

Thermo Scientific™ DNase I Solution (2500 U/mL)

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

EDGE
15
Promotions Available

Thermo Scientific™ Brij™-35, 30% Solution

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

EDGE