PCR and qPCR

Applied Biosystems™ TaqMan® Gene Expression Assay, SM

TAQMAN GENE EXPRESSION ASSAY250 reactions, Reporter Dye: FAM

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. SSIII 1-STEP QRT-PCR 500500 reactions

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR) 2000 RXN REVERSE TRANSCRIPTION REAGENTS (10 PACK)

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

Contains everything required for successful PCR amplification in a convenient package—all components are premixed and premeasured 50ML AMPLITAQ GOLD 360 MASTER MIX

Invitrogen™ SuperScript™ IV Reverse Transcriptase

A proprietary MMLV mutant with superior robustness and reliability in RT reactions. 40000UNIT SuperScript IV Reverse Transcriptase

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. X2 POWRUP SYBR MASTER MIX,2X5 ML

Invitrogen™ SuperScript™ II Reverse Transcriptase

An improved version of SuperScript RT 10 KU SUPERSCRIPT II STORE AT -20°C

Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes 50 RXN HIGH CAPACITY RNA-TO-CDNA KIT 50REACTIONSStore at -20 C

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes X200 MICROAMP, FAST OPTICAL 96-WELL REACTIONplate with barcode, 0.1mL

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis Syste

Invitrogen™ Water-Saturated Phenol, pH 6.6

Ready-to-use, safe alternative to preparing crystalline phenol solutions 100 ML WATER SATURATED PHENOL, PH 6.6 (RAISE TOpH 7.9 with includedbuffer) 100mL Store at 4°C

Invitrogen™ MultiScribe™ Reverse Transcriptase

A recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays 100 UL MULTISCRIBE REVERSE TRANSCRIPTASE 100µLStore at -20 C

Applied Biosystems™ VeritiLink™ Remote Management Software

Easy-to-install software provides secure, remote management for Veriti Thermal Cyclers from anywhere on network REMOTE MANAGEMENT SOFTWARE VERITI(R) EASY-TO-INSTAll

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. PHUSION HF MASTER MIX 500 RXNS

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA 2 RXN HIGH CAPACITY CDNA REVERSE TRANSCRIPTIONkit 200reactionsStore at -20 C

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. 1 ML SYBR GREEN PCR MASTER MIX, MINI PACK 1MLStore at -20 C

Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96-well)

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System. 96 WELL LHS SYS DYE CAL PLATECalibration Kit

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Oligo(dT) 20 Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C OLIGO DT(20) PRIMER

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 96-WELL REACTION PLATE

Applied Biosystems™ ProFlex 96-well PCR System, Range: 10-80μL

Combines the reliability and performance of Applied Biosystems™ instruments with the flexible configuration and control features that adapt to your research needs PCR system ProFlex 96 well (0.2mL VeriFlex,

Applied Biosystems™ Random Hexamers (50mM)

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase 1 SET Random Hexamers (50um) 1kit Store at -20 C

Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications. 40 RXN GENOTYPING MASTER MIX, MINI-PACK (1ML)40reactions Store at 4°C

Applied Biosystems™ TaqMan® Gene Expression Assay, SM

KIT,TQMN GENE EX ASSAYS, SMreactions, Reporter Dye: FAM

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 2500 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NO

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation AMPLITAQ GOLD 12X250UGold Buffer + MgCl2 Sol., 3000 units

Applied Biosystems™ TaqMan™ Advanced miRNA Assays

Enable highly sensitive and specific quantification of mature miRNAs using qPCR. TaqMan MicroRNA Assays

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

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