PCR and qPCR

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. 100 RXN PHUSION HIGH-FIDELITY PCR MASTER MIX GCbuffer formulation (2 x 1.25mL), 2X Master Mix

Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes 50 RXN HIGH CAPACITY RNA-TO-CDNA KIT 50REACTIONSStore at -20 C

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 5000 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NOAmpErase UNG, 50mL 5000reactions Store at 4°C

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis System

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

4LT NUCLEASE-FREE WATER 4X1 LITER

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes X200 MICROAMP, FAST OPTICAL 96-WELL REACTIONplate with barcode, 0.1mL

Invitrogen™ SuperScript™ IV Reverse Transcriptase

A proprietary MMLV mutant with superior robustness and reliability in RT reactions. 40000UNIT SuperScript IV Reverse Transcriptase

Fisherbrand™ 96-Well Low-Profile, Skirted PCR Plates, Natural

Fit most thermal cyclers X25 Fisherbrand PCR plate 96-wells, full skirt, PP, blue - FB-0800/B

Invitrogen™ Oligo(dT) 20 Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C OLIGO DT(20) PRIMER

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA KIT, HIGH CAPACITY CDNA RT1000 reactions

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate with Barcode

Optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification MicroAmp(R) Optical 96-well Reaction PlatewithBarcode (code 128), 25-Pack, 500/Pkg

SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. SSIII 1-STEP QRT-PCR 500500 reactions

PowerUp™, SYBR™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. 50ML POWRUP SYBR MASTER MIX

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 96-WELL REACTION PLATE

Applied Biosystems™ TaqMan® Gene Expression Assay, SM

TAQMAN GENE EXPRESSION ASSAY250 reactions, Reporter Dye: FAM

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps X2500 Platinum SuperFi DNA Polymerase

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ MultiScribe™ Reverse Transcriptase

A recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays 100 UL MULTISCRIBE REVERSE TRANSCRIPTASE 100µLStore at -20 C

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. ezDNase

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR) 2000 RXN REVERSE TRANSCRIPTION REAGENTS (10 PACK)

Invitrogen™ SuperScript™ III Reverse Transcriptase

Proprietary mutant of SuperScript II RT 2 KU REVERSE TRANSCRIPTASE SUPERSCRIPT(TM) IIIStore at -20C

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix

TaqMan™ Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes). TAQMAN FAST ADVANCED MASTER MIX (1 X 50 ML)

Applied Biosystems™ MicroAmp™ Cap Installing Tool

MicroAmp™ Cap Installing Tool MicroAmp® Cap Installing Tool (Handle)

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps 100 RXNS Platinum Superfi Master Mix

Thermo Scientific™ Thermo-Fast™ 96-Well Full-Skirted Plates

96-well full-skirted PCR automation compatible plate for use in PCR and qPCR applications. X25 THERMOFAST SK 96X0,2ML BLUEblue (VE=25Stck.)

Applied Biosystems™ Random Hexamers (50mM)

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase 1 SET Random Hexamers (50um) 1kit Store at -20 C

TaqMan™ TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription. TAQMAN ADV MICRORNA CDNA SYN (50 RXNS)

Thermo Scientific™ ABgene™ Thermo-Fast™ 96-Well Semi-Skirted PCR Plates

Patented segmented design allows plate to be cut into 24- and 48-well sections X25 THERMOFAST SSK 96X0,2ML GREEN(VE=25Stck.)

Merck™ Novagen™ Rosetta™ 2(DE3)pLysS Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli. 1ML Rosetta 2(DE3)pLysS Competent Cells

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