PCR and qPCR

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA KIT, HIGH CAPACITY CDNA RT1000 reactions

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes X200 MICROAMP, FAST OPTICAL 96-WELL REACTIONplate with barcode, 0.1mL

Invitrogen™ Oligo(dT) 20 Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C OLIGO DT(20) PRIMER

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. POWER UP SYBR MASTER MIX 5X5 ML

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins 1 ML PROTEINASE K SOLUTION (20 MG/ML) 1ML STOREat -20 C

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR) 2000 RXN REVERSE TRANSCRIPTION REAGENTS (10 PACK)

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 96-WELL REACTION PLATE

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 5000 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NOAmpErase UNG, 50mL 5000reactions Store at 4°C

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. SSIII 1-STEP QRT-PCR 500500 reactions

Invitrogen™ SuperScript™ III Reverse Transcriptase

Proprietary mutant of SuperScript II RT 2 KU REVERSE TRANSCRIPTASE SUPERSCRIPT(TM) IIIStore at -20C

Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes 50 RXN HIGH CAPACITY RNA-TO-CDNA KIT 50REACTIONSStore at -20 C

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. X4 Routine PCR, PCR master mixes, DreamTaq(TM)

Axygen™ 8-Strip PCR Tubes, 0.2mL

Available in a variety of colors for easy sample identification. Axygen™ 8-Strip PCR Tubes, 0.2mL are constructed of sturdy polypropylene. Each non-sterile, conical tube features thin and consistent wall thickness. Sold with flat or domed caps to prevent sample loss or without caps. X125 0,2ML 8 STRIP TUBE RED

Invitrogen™ SuperScript™ II Reverse Transcriptase

An improved version of SuperScript RT 2 KU RNASE H SUPERSCRIPT(TM) II STORE AT -20C

Applied Biosystems™ ProFlex 96-well PCR System, Range: 10-80μL

Combines the reliability and performance of Applied Biosystems™ instruments with the flexible configuration and control features that adapt to your research needs PCR system ProFlex 96 well (0.2mL VeriFlex,

Eppendorf™ Polypropylene PCR Tubes

Ensure efficient and homogenous heat transfer with these thin-walled polypropylene tubes. Eppendorf™ Polypropylene PCR Tubes are easy to open, but provide tight sealing to prevent evaporation in PCR. X120 PCR 0.2ml 8-Tubes Strip, PCR Clean, bagged/10(960 tubes) - individual hinged lid

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Storage Requirements: Store at room temperature. NUCLEASE-FREE WATER 100 MLAmbion® Nuclease-free water has not been treated

Applied Biosystems™ TaqMan® Gene Expression Assay, SM

TAQMAN GENE EXPRESSION ASSAY250 reactions, Reporter Dye: FAM

Invitrogen™ SuperScript™ IV Reverse Transcriptase

A proprietary MMLV mutant with superior robustness and reliability in RT reactions. 40000UNIT SuperScript IV Reverse Transcriptase

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. 50 ML SYBR GREEN PCR MASTER MIX, BULK PACK 50MLStore at -20 C

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

Contains everything required for successful PCR amplification in a convenient package—all components are premixed and premeasured 50ML AMPLITAQ GOLD 360 MASTER MIX

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis Syste

Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance. X100 UNITS PHUSION HOT START II HIGH-FIDELITY DNA

Eppendorf™ Polypropylene DNA LoBind Tubes

Maximize sample recovery of nucleic acids by significantly reducing sample-to-surface binding with these tubes. Eppendorf™ Polypropylene DNA LoBind Tubes are ideal for sample preparation and long-term storage. X250 DNA LoBind tube 2ml PCR clean

Applied Biosystems™ SimpliAmp™ Thermal Cycler

Small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR SIMPLIAMP THERMAL CYCLER: 3 INDEPENDENT TEMP ZONES

Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates, Semi-skirted

Single use polycarbonate/polypropylene plates allowing trained specialists to perform PCR and cycle sequencing experiments. Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates are used for PCR in applications including training, routine, and research laboratories in the bioscientific, industrial, and chemical fields X25 TWINTEC PCR PLATE 96 LOBIND SEMISKIRTED, CLEAR

Applied Biosystems™ Random Hexamers (50mM)

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase 1 SET Random Hexamers (50um) 1kit Store at -20 C

Thermo Scientific™ 0.2 mL Strip Tubes

Optimize PCR and qPCR with these 0.2 mL strip tubes, available as 8 tubes per strip in several colors or 12 tubes per strip. X120 STRIPS OF 8 DOMED CAPS ST

Applied Biosystems™ StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well)

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System. 48 WELL LHS SYS DYE CAL PLATECalibration Kit, 1 Kit

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