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Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads
Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.
| Particle Size | 1 μm |
|---|---|
| Ligand Type | Protein A/G |
| Type | Magnetic Affinity Bead |
| Target | Antibody |
| Content And Storage | Upon receipt store at 4°C. Product shipped with an ice pack. |
| Protein Form | Recombinant |
| Form | Liquid |
| Product Line | Pierce |
Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)
Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.
| Content And Storage | Store at Room Temperature |
|---|---|
| Form | Powder |
| Product Type | Detergent |
| Reagent Type | Detergent (Pure) |
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
| Content And Storage | Store at room temperature. |
|---|---|
| Column Type | Empty Column, Polyethylene Filter |
| Product Line | Pierce |
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Invitrogen™ DynaMag™-96 Side Magnet
Designed for both automation platforms (e.g., Tecan) and manual workflows, but is compatible with PCR-strips and 96-well plates (200 μL), non-skirted and half-skirted.
Thermo Scientific™ DNase I Solution (1 unit/μL), RNase-free
Cleave and degrade unwanted single- and double-stranded DNA
Thermo Scientific™ DNase I Solution (2500 U/mL)
Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.
Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown
Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.
| Content And Storage | Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C. |
|---|---|
| Final Product Type | His-Tagged Protein |
| Color | Brown |
| Binding Property | 1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads |
| Material | Magnetic Polystyrene |
| Concentration | 40 mg/mL |
| For Use With (Application) | Isolation of His-tagged Recombinant Proteins |
| High-throughput Compatibility | Yes |
| Regulatory Status | For Research Use Only |
| Ligand Type | Nitrilotriacetic acid (NTA) |
| Type | Magnetic Beads |
| Target | His-tagged proteins |
| Format | Magnetic beads |
| For Use With (Equipment) | KingFisher Sample Purification System, DynaMag magnets |
| Sample Type | Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts |
| Diameter (Metric) | 1 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Shelf Life | 36 months from date of manufacture |
| Product Line | Dynabeads |
Invitrogen™ Dynabeads™ M-270 Epoxy
Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.
| Shipping Condition | Room Temperature |
|---|---|
| Type | Epoxy Bead |
| Purity or Quality Grade | Research Grade |
| Format | Freeze Dried |
| Surface Functionality | Epoxy |
| Sample Type | Any Sample Type |
| Diameter (Metric) | 2.8 μm |
| Shelf Life | 24 months from date of manufacture |
| Elution Volume Min | 5 μL |
| Product Line | DYNAL, Dynabeads |
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
| Particle Size | 45 to 165 μm |
|---|---|
| Stationary Phase | Streptavidin |
| Type | Agarose |
| Target | Biotin-Tagged Molecule |
| Content And Storage | Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Sufficient For | Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin) |
| Product Line | Pierce |
Gibco™ Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation
Dynabeads Mouse T-Activator CD3/CD28 beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-mouse CD3 and anti-mouse CD28 antibodies and intended for ex vivo activation and expansion/proliferation of mouse T cells.
| Shipping Condition | Ambient Temperature |
|---|---|
| Clonality | Monoclonal |
| Target Species | Mouse T-cells |
| Host Species | Hamster |
| Description | Monoclonal antibodies covalently bound to DynaBeads magnetic Beads |
| Surface Functionality | Epoxy |
| Isolation Technology | Magnetic Beads |
| Color | Brown |
| Material | Magnetic Polystyrene |
| Concentration | 4 x 10 7 Beads/mL |
| For Use With (Application) | Activation and Expansion of T-cells |
| Cell Type | Mouse T-cells |
| Regulatory Status | For Research Use Only |
| Reactivity | Mouse |
| For Use With (Equipment) | DynaMag™ Magnet |
| Product Type | Cell Expansion and Activation Kit |
| Diameter (Metric) | 4.5 μm |
| Sample Type | Cell Cultures, Lymph Node, Spleen |
| Shelf Life | 36 months from date of manufacture |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 4.5 μm (CV <5%) |
| Product Line | DynaBeads |