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Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

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Competent Cells

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

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Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.

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Specifications

Nucleic Acid Labeling and Detection

Shipping Condition	Room Temperature
No. of Reactions	1000 Assays (200 μL assay volume)
For Use With (Equipment)	Microplate Reader
Quantity	1 kit
Product Line	Quant-iT
Detection Method	Fluorescence

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Invitrogen™ Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>300X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

Thermo Scientific™ GeneJET Genomic DNA Purification Kit

Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.

Pricing and Availability
Specifications

DNA Extraction and Purification

Column Type	Spin Column
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion Plus DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	>100X
Size (Final Product)	20 kb or less
Reaction Format	Ready to use master mix
Reaction Speed	15 to 30 sec./kb
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

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Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Pricing and Availability
Specifications

DNA Cloning and Mapping and Mutagenesis

Shipping Condition	Ambient, Wet Ice, Dry Ice
Content And Storage	Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step	DNA Assembly
Number of Fragments	Up to 6 fragments
Product Type	HiFi Master Mix
Control Type	Positive Control
Cloning Method	Seamless cloning
For Use With (Application)	Seamless Cloning & Genetic Assembly
Product Line	GENEART™

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Invitrogen™ Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is an optimized lipid-based transfection reagent for CRISPR-Cas9 protein delivery, providing the cleavage efficiency of electroporation with simplicity and scalability.

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Specifications

Transfection Reagents

Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Product Type	Transfection Reagent
Transfection Technique	Lipid-based Transfection
Sample Type	Protein,Ribonucleoprotein,Cas9 Ribonucleoprotein,Cas9,Plasmid DNA
Classification	Animal Origin-Free
Serum Compatible	Yes
For Use With (Application)	Transfection
Product Line	CRISPRMAX™, Lipofectamine™
Cell Type	Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells

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Thermo Scientific™ Oligo(dT)₁₈ Primer

Thermo Scientific Oligo(dT)₁₈ Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Pricing and Availability
Specifications

Oligonucleotides

Shipping Condition	Dry Ice
Primer Length	18-mer
Form	Liquid
Product Type	Primer
Primer	Oligo dT
Concentration	0.5 μg/μL
For Use With (Application)	cDNA Synthesis

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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase™, TaqMan™

Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)

Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.

Pricing and Availability
Specifications

Isothermal Amplification Reagents

Shipping Condition	Dry Ice
Polymerase	phi29 DNA Polymerase
Product Type	Stand-alone enzyme
Reaction Time	4 hours
Concentration	10 U/μL
Optimal Reaction Temperature	30°C
For Use With (Application)	MDA-WGA,RCA

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Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.

Pricing and Availability

DNA Extraction and Purification

Gibco™ ExpiFectamine™ CHO Transfection Kit

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.

Pricing and Availability
Specifications

Transfection Reagents

Shipping Condition	Room Temperature
Promoter	pcDNA™ 3.4
Cell Line	ExpiCHO-S™, CHO-S, SFM Adapted
Format	Kit
Product Type	CHO Transfection Kit
Transfection Technique	Lipid-based Transfection
Classification	Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Product Line	ExpiFectamine™
Cell Type	Ovarian Cells

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Dry Ice
Content And Storage	• PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark.
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	8 hr., Assembled Reaction
Polymerase	Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	Applied Biosystems™

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TaqMan™ Control Genomic DNA (human)

Conveniently packaged in a PCR-ready concentration for use as a control template in PCR reactions

Pricing and Availability

Nucleic Acid Controls

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Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Quant-iT™ dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)