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Cytiva HiTrap™ HIC Sepharose™ Selection Kit
Use for laboratory and intermediate process-scale separations and final-step purifications. Cytiva Life Sciences™ HiTrap™ HIC Sepharose™ Selection Kit takes full advantage of hydrophobic interaction chromatography providing highest resolution.
Cytiva HiTrap™ MabSelect™ SuRe Prepacked Columns
Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Binding Capacity | ≈35mg Human lgG/mL at 2.4 min. residence time |
| For Use With (Application) | Chromatography System, Manual |
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
| Particle Size | 34μm |
|---|---|
| Buffer | 6M Guanidine Hydrochloride, 8M Urea |
| Ligand Type | Iminodiacetic Acid |
| Height (Metric) | 25mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 13 |
| Product Type | HiTrap chelating HP column |
| Column Type | Affinity Column |
| For Use With (Application) | IMAC and subsequent purification of Polyhistidine tagged Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 3 bar |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ Benzamidine FF (high sub) Columns
Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).
| Particle Size | 90 μm |
|---|---|
| Ligand Type | >12μM p-aminobenzamidine/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 2° to 8°C |
| pH Range | 2 to 8 |
| Product Type | Benzamidine FF Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Sample Type | Serine Proteases such as Thrombin |
| Material | Polypropylene |
| For Use With (Application) | Removal or purification of Serine Proteases such as Thrombin |
| Support Type | 4% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ IMAC Columns
For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion
| Flow Rate | <4 mL/min. |
|---|---|
| Content And Storage | 4°C to 30°C |
| pH Range | 3 to 12 |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system |
| Material | Polypropylene |
| For Use With (Application) | For optimizing purification of His-tagged Proteins |
| Max. Pressure | 42 psi |
Cytiva HiTrap™ Capto™ IEX Selection Kit
Designed for use with syringe, peristaltic pump, or chromatography systems such as AKTA design for convenient media screening and optimization studies
Cytiva HiTrap™ Protein L Prepacked Columns
For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs
| Particle Size | 85μm |
|---|---|
| Ligand Type | Recombinant Protein L (E. coli), mammalian free |
| Flow Rate | <500 cm/hr. |
| Height (Metric) | 20mm |
| Content And Storage | 2° to 8°C |
| pH Range | 2 to 10 |
| Product Type | HiTrap Protein L column |
| Column Type | Affinity Column |
| Material | Polypropylene |
| For Use With (Application) | Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs |
| Support Type | Highly Cross-linked Agarose |
| Max. Pressure | 5 bar |
| Height (English) | 0.78 in. |
Cytiva HiTrap™ IgY Purification Column
Developed for fast, easy purification of IgY from chicken egg yolk
Cytiva HiTrap™ IgG Sepharose™ FF
For use in chromatography. Cytiva Lifescience HiTrap™ IgG Sepharose™ FF is stable to all commonly used aqueous buffers.
Cytiva HiTrap™ SP Sepharose XL IEX Columns
Optimized for fast, convenient small-scale protein capture
| Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
|---|---|
| Description | HiTrap SP XL |
| For Use With (Application) | HiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography. |
Cytiva HiTrap™ rProtein A FF Columns
Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.
| Content And Storage | 4°C to 30°C, 20% Ethanol |
|---|---|
| For Use With (Equipment) | For syringe, peristaltic pump or a chromatography system |
| Binding Capacity | ≈50 mg Human IgG/mL |
| For Use With (Application) | Chromatography System, Manual |
Cytiva HiTrap™ Blue HP Columns
For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins
| Particle Size | 34 μm |
|---|---|
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 4 to 12 |
| Product Type | Blue HP Columns |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Sample Type | Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins |
| Material | Polypropylene |
| For Use With (Application) | Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
| Particle Size | 45 to 165 μm |
|---|---|
| Ligand Type | Phenyl |
| Height (Metric) | 25 mm |
| pH Range | 3 to 13 |
| Product Type | HiTrap Phenyl FF (Low Sub) |
| Material | Polypropylene |
| Storage Requirements | 4°C to 30°C, 20% Ethanol |
| For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
| Support Type | 6% Cross-linked Agarose |
| Media Type | Phenyl Sepharose 6 Fast Flow (low sub) |
| Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
| Height (English) | 0.984 in. |
| Particle Size | 90 μm (d50V) |
|---|---|
| Ligand Type | Diethylaminoethyl |
| Content And Storage | 4°C to 30°C in 20% Ethanol |
| Matrix | Highly cross-linked agarose with dextran surface extender |
| pH | 2 to 9 |
| Column Material | Polypropylene |
| Particle Size | 45 to 165 μm |
|---|---|
| Ligand Type | Butyl |
| Height (Metric) | 25 mm |
| pH Range | 3 to 13 |
| Product Type | HiTrap Butyl FF |
| Material | Polypropylene |
| Storage Requirements | 4°C to 30°C, 20% Ethanol |
| For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
| Support Type | 4% Cross-linked Agarose |
| Media Type | Butyl Sepharose 4 Fast Flow |
| Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
| Height (English) | 0.984 in. |