LAMP and Other Isothermal Amplification Technologies

Nucleic acid (RNA and DNA) amplification products designed to amplify targeted material at a constant temperature as opposed to thermocylcing. Products include loop-mediated isothermal amplification (LAMP) assays as well as other isothermal amplification reagents, kits, and buffers. Products are well suited for detecting a variety of pathogens from a broad array of source material, including animal, plant, and environmental samples.

Isothermal Amplification Reagents (19)

LAMP Assays (2)

1 – 9 of 9 results

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Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)

Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	phi29 DNA Polymerase
Product Type	Stand-alone enzyme
Reaction Time	4 hours
Concentration	10 U/μL
Optimal Reaction Temperature	30°C
For Use With (Application)	MDA-WGA,RCA

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Invitrogen™ Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Lyo-ready Bst DNA Polymerase
Product Type	Stand-alone enzyme
Reaction Time	≤10 minutes
Optimal Reaction Temperature	65°C
For Use With (Application)	LAMP,RT-LAMP

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Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	EquiPhi29 DNA Polymerase
Format	Kit
Form	Liquid
Product Type	DNA Amplification Kit
Reaction Time	Up to 2 hours
Research Category	Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection
Optimal Reaction Temperature	42°C
For Use With (Application)	RCA,MDA-WGA

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Thermo Scientific™ EquiPhi29™ DNA Polymerase

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	EquiPhi29 DNA Polymerase
Product Type	Stand-alone enzyme
Reaction Time	2 hours
Concentration	10 U/μL
Optimal Reaction Temperature	42°C
For Use With (Application)	MDA-WGA,RCA

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Invitrogen™ SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Bst DNA Polymerase
Includes Label or Dye	SYTO 9 Green Fluorescent Nucleic Acid Stain
Reverse Transcriptase	SuperScript IV
Product Type	Master Mix
Reaction Time	5 to 15 minutes
Concentration	2X
Optimal Reaction Temperature	65°C
For Use With (Application)	LAMP,RT-LAMP

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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Form	Liquid
Research Category	Nucleic Acid Amplification

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Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)

Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.

Pricing and Availability

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Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

Pricing and Availability

Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2

The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.

Pricing and Availability
Specifications

Includes	LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA
Detection Limit	<250 copies/25 μL (Direct LAMP) <100 copies/25 μL (Purified RNA-based LAMP)
Bead Type	Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads
For Use With (Equipment)	Heating block
Enzyme	SuperScript IV & proprietary Bst-derived DNA polymerase
Formulation	Liquid. 5 components included in the kit.
Sample Type	Saliva or Nasopharyngeal/Nasal Swab
Color	During reaction color changes from purple to blue
Sample Volume	Range of sample inputs
Key Functions	Detects SARS-CoV-2 presence
For Use With (Application)	SARS-CoV-2 surveilance
Detection Method	Colorimetric readout

LAMP and Other Isothermal Amplification Technologies

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