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Protein Purification Reagents and Kits

Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 123 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
3

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

Promotions are available
4

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

Promotions are available
5

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
6

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.   Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Promotions are available
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
7

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Promotions are available
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
8

Cytiva HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

Promotions are available
9

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL.  Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
11

Cytiva Glutathione Sepharose™ 4B Media

Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.

Promotions are available
Particle Size 90 μm
pH Range 4 to 13
Material Glutathione Sepharose 4 B
12

Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

13

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes.   Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Promotions are available
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
14

Cytiva Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

15

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose