Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Pricing and Availability
Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Pricing and Availability

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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Stationary Phase	Anti-DYKDDDDK monoclonal antibodies
Product Type	Affinity Resin

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability

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Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

Pricing and Availability
Specifications

Type	Agarose
Stationary Phase	Ni-NTA
Content And Storage	The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form	Suspension
Column Type	Affinity Column

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Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Pricing and Availability
Specifications

Stationary Phase	Divalent Nickel, Metal Chelate
Type	Chromatography Cartridge
Target	His-Tagged Protein
Content And Storage	Store between 4°C and 8°C. Do not freeze.
Column Type	Affinity, Agarose Resin
Product Line	HisPur™

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Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

Pricing and Availability
Specifications

Stationary Phase	Proprietary Ligand
Column Type	Proprietary Resin, Affinity
Purification Target	Detergent Removal

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Pricing and Availability
Specifications

Flow Rate	<20 mL/min.
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.6 in.
Description	HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Diameter (Metric) Inner	16 mm
Material	Polypropylene
Support Type	6% Cross-linked Agarose

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Particle Size	50 to 150 μm
Ligand Type	Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase	Agarose
Type	Bulk Resin
Target	Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format	Bottle
Description	4% agarose, crosslinked
Final Product Type	Affinity chromatography equipment
Protein Form	Tetrameric protein
Form	Resin Slurry
Binding Capacity	20 mg/mL resin
Column Type	Suspension
Concentration	50% suspension

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Invitrogen™ ProBond™ Nickel-Chelating Resin

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

Pricing and Availability
Specifications

Type	Resin
Stationary Phase	Nickel-Chelating
Content And Storage	The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
Form	Suspension
Product Line	ProBond™

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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Pricing and Availability
Specifications

Stationary Phase	Divalent Nickel, Metal Chelate
Type	Agarose
Target	His-Tagged Protein
Content And Storage	Store at 4°C
Format	Bottle
Form	Liquid Suspension
Column Type	Affinity, Agarose Resin
Capacity (Metric)	20 mg His-Tagged GFP Binding per mL Resin
Product Line	HisPur™

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Pricing and Availability
Specifications

Particle Size	50 to 150 μm
Ligand Type	Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase	Agarose
Type	Column
Target	Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Description	4% agarose, crosslinked
Final Product Type	Affinity chromatography equipment
Protein Form	Tetrameric protein
Form	Resin Slurry
Binding Capacity	20 mg/mL resin
Quantity	5 mL

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Thermo Scientific™ AminoLink™ Coupling Resin

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Pricing and Availability
Specifications

Stationary Phase	Aldehyde
Type	Resin
Target	Antibody, Proteins through Primary Amine
Content And Storage	Store at 4°C.
Format	Bottle
Column Type	Affinity, Agarose Resin
Product Line	AminoLink™

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's.

Pricing and Availability
Specifications

Particle Size	65 ±10 μm
Shipping Condition	Wet Ice
Ligand Type	KappaXP affinity ligand
Flow Rate	150 to 300 cm/hr. (Up to 2 bar)
Stationary Phase	KappaXP
Type	Affinity Matrix
Content And Storage	Store in refrigerator (2°C to 8°C).
Matrix	Agarose-based
Product Line	CaptureSelect™

Thermo Scientific™ CaptureSelect™ FcXL Affinity Matrix

Thermo Scientific™ CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG.

Pricing and Availability
Specifications

Ligand Type	FcXL affinity ligand
Flow Rate	50 to 200 cm/hr. (Up to 2 bar)
Target	Fc of IgG, CH3 domain
Matrix	Agarose based, aldehyde activated
Form	Liquid Suspension
Binding Capacity	25 to 35 g IgG/L
For Use With (Application)	IgG purification

Protein Purification Reagents and Kits

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