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The mMESSAGE mMACHINE T7 ULTRA Transcription Kit generates RNA transcripts with higher protein yields due to the combination of the anti-reverse cap analog (ARCA) with patented transcription reagents.
Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins
mMESSAGE mMACHINE kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end.
The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit.
Shipping Condition | Dry Ice |
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Promoter | Proprietary |
Format | Kit |
Sub Cellular Localization | Cytoplasm |
Product Type | Sendai Reprogramming Kit |
Delivery Type | Sendai virus |
For Use With (Application) | Stem Cell Engineering & Reprogramming |
Product Line | CytoTune™ |
Cell Type | iPSC |
in vitro synthesis of large amounts of capped RNA
Cap analog for in vitro transcription for capped transcripts generation
Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines
The MEGAclear kit can be used to remove nucleotides, short oligonucleotides, proteins, and salts from RNA. The RNA recovered can be used for any application that requires high purity RNA. Recover from 1 ng to 500 ÎĽg of RNA.
Ultra-high yield technology for in vitro transcription.
Maximize the yield of short RNA transcripts
Obtain 10–50 times yield of conventional in vitro transcription reactions.
For 5' capped RNA molecules synthesis as part of in vitro transcription reactions
Generate RNA transcripts with higher protein yields
Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations
Promoter | T7 |
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Content And Storage | T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature. |
Format | Kit |
Final Product Type | Synthetic mRNA,mRNA |
Sample Type | mRNA,Synthetic mRNA |
Yield | 2-6 ÎĽg |
Starting Material | PCR Products, Linearized DNA plasmid |
For Use With (Application) | In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products |
Product Line | Ambion™, MAXIscript™ |
The CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit is the first off-the-shelf reprogramming system designed for clinical and translational research. It delivers efficient, integration-free reprogramming of somatic cells into induced pluripotent stem cells.