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Invitrogen™ MAXIscript™ T7 Transcription Kit

Code produit 10762867
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Product Type:
T7 In Vitro Transcription Kit
T7 In Vitro Transcription Kit with Manual
Quantity:
100 Reactions
30 Reactions
Conditionnement:
Each
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Code produit Product Type Quantity unitSize
10762867 T7 In Vitro Transcription Kit with Manual 100 Reactions Each
10430355 T7 In Vitro Transcription Kit 30 Reactions Each
10035164 T7 In Vitro Transcription Kit 100 Reactions Each
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Code produit 10762867 Fournisseur Invitrogen™ Code fournisseur AM1314M
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Description

Includes

T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

The Ambion™ MAXIscript™ T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions. The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/μg in just 10 minutes. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase™
  • All kits contain quality-tested DNase/RNase-free reagents
  • RNA Yield: 2 to 6μg

TRUSTED_SUSTAINABILITY

Spécification

Content And Storage T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 100 Reactions
Product Line Ambion, MAXIscript
Product Type T7 In Vitro Transcription Kit with Manual
Quantity 100 Reactions
Promoter T7
Yield 2-6 μg
Starting Material PCR Products, Linearized DNA plasmid
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For Research Use Only. Not for use in diagnostic procedures.

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