Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (14,470)

Singleplex and Multiplex Bead Based Immunoassays (1,390)

ELISA Substrates and Reagents (1,232)

1 – 15 of 13,877 results

Invitrogen™ Cytokine 25-Plex Human Panel

Specifically designed for quantifying human cytokines and chemokines in serum, plasma, and tissue culture supernatant

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Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit

Assay Urea Nitrogen (BUN) levels in Serum, Plasma, Urine, Saliva and Tissue Culture Media.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Colorimetric Detection Kit
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at 2–8°C.
For Use With (Equipment)	Microplate Reader
Gene ID (Entrez)	Non-Gene
Gene Symbol	Non-Gene
Target Kit Named	Urea Nitrogen (BUN)

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Invitrogen™ Human IL-8 ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.1%
Intraassay CV	3.9
Gene Symbol	GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time	2 hrs 30 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6 to 1000 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hrs 20 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	<5 pg/mL
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Pricing and Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	Alkaline Phosphatase
Detection Method	Colorimetric

Invitrogen™ Human TNF alpha Uncoated ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	CANDF6,IL-17 F,ML-1,ML1
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01375
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7124
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a, tnf-alpha

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Invitrogen™ Human IL-6 ELISA Kit

ELISA

Pricing and Availability
Specifications

Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Description	Human IL-6 ELISA Kit
Precision	Intra-assay CV <10%; inter-assay CV <10%

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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pricing and Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

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Invitrogen™ Human Tau (Total) ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.5%
Intraassay CV	4.6
Incubation Time	4 hr.
Gene Symbol	DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT
Total Assay Time	4 hrs
Sample Volume	Cerebrospinal Fluid 50 μL, Supernatant 50 μL
Assay Sensitivity	<10 pg/mL
Storage Requirements	2°C to 8°C
Concentration	31 to 2000 pg/mL
Assay Range	31 to 2000 pg/mL
Marker Gene	Tau
Test Time	1 hrs 20 mins
Accession Number	P10636
Regulatory Status	RUO
Target	Tau
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	4137
Sample Type	Cerebrospinal Fluid,Supernatant
Label or Dye	HRP

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Invitrogen™ Mouse Amyloid beta 42 ELISA Kit

ELISA

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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

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Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.

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Invitrogen™ Human Albumin ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Assay	Solid-phase sandwich ELISA
Target	Human Serum Albumin
Specificity	This ELISA pair antibody detects human Albumin. Other species not determined.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	60 pg/mL to 12,000 pg/mL
Interassay CV	<12%
Gene Symbol	HSA,PRO0883,PRO0903,PRO1341
Storage Requirements	2°C to 8°C
Assay Range	4.915 to 1200 ng/mL
Accession Number	P02768
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	213
Sample Type	Plasma,Serum,Supernatant
Synonym	GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341

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Invitrogen™ Human Tau (Phospho) [pS199] ELISA Kit

ELISA

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ELISA Systems™ Hazelnut Residue Detection kit for Food Allergen Testing

Rapidly detect and quantify hazelnut material in food and environmental samples.

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Invitrogen™ Human Cortisol Competitive ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Description	Cortisol Competitive ELISA Kit
Interassay CV	8.1%
Intraassay CV	8.8
Total Assay Time	1 hrs 30 mins
Sample Volume	Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL
Assay Sensitivity	17.3 pg/mL
Storage Requirements	-20°C
Research Discipline	Inflammation
Assay Range	100 to 3200 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hrs 20 mins
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Cortisol
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye	HRP

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