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  6. Invitrogen™ ZO-1 Monoclonal Antibody (R26.4C), eBioscience™, Invitrogen™

Invitrogen™ ZO-1 Monoclonal Antibody (R26.4C), eBioscience™, Invitrogen™

Product Code. p-7069666
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Quantity:
100 μg
25 μg
Unit Size:
100µg
25µg
Product Code. Quantity unitSize
15547676 25 μg 25µg
15557676 100 μg 100µg
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Product Code. 15547676

Brand: Invitrogen™ 14977680

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Description

Rat Monoclonal Antibody

Description: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression. Applications Reported: This R26.4C antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry. Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay interest.

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
TRUSTED_SUSTAINABILITY

Specifications

Antigen ZO-1
Applications Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone R26.4C
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene TJP1
Gene Accession No. O97758, P39447
Gene Alias DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
Gene Symbols TJP1
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 21872, 292994, 403752
Target Species Mouse, Rat, Canine
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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