QIAGEN QIAcard FTA DMPK

QIAcard FTA DMPK Cards for Drug Metabolism Pharmacokinetic enable microvolume sampling with only 10 to 20 μL per sample and are designed for use with blood samples. The cards can also be used with colorless samples such as plasma, cerebrospinal fluid and urine.

QIAcard FTA DMPK-A and QIAcard FTA DMPK-B contains impregnated chemicals enabling protein denaturation to inactivate endogenous enzymes. Cell lysis releases endogenous cellular materials onto card. Stabilization of DNA allows resampling of blood spot for pharmacogenomics.Impregnated chemicals may interfere with mass spectrometry detection (e.g. ion suppression).

QIAcard FTA DMPK-C contain pure cellulose. No impregnated chemicals to interfere with analysis. Proteins are not denatured so cards may be better suited for protein based biomolecules and antibody studies.

Analysis:

Analysis primarily with HPLC or UHPLC followed by MS/MS detection, direct desorption MS techniques such as DESI. Methanol or aqueous/organic mixtures have been shown to work well for small molecules and aqueous buffers are recommended for proteins.

Select a good solvent for the analyte while maintaining compatibility with the HPLC conditions to be used. The choice of DMPK card depends on many factors such as the analyte chemical structure, extraction solvent and analysis workflow.