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Invitrogen™ Vybrant™ FLICA Caspase Apoptosis Assay Kits for flow cytometry

Detect apoptosis via flow cytometry with Vybrant FLICA Caspase Assays that detect poly and caspase-3/7/8 activities.

Brand:  Invitrogen™ V35118

2857.55 NOK valid until 2025-06-30
Use promo code "24074" to get your promotional price.



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Product Code. 10003352

  • 4385.00 NOK / Each

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Description

Description

Quickly detect caspase-mediated apoptosis by flow cytometry with Vybrant FAM caspase assay kits for poly-caspases and caspases 3 and 7. The Vybrant FAM caspase assay kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity. Specifically, these caspase assay kits contain engineered FLICA substrates FAM-VAD-FMK (for poly-caspases), FAM-DEVD-FMK (for caspase-3/7), and FAM-LETD-FMK (for caspase-8). They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by flow cytometry.

The Vybrant FAM caspase assay kits for flow cytometry employ a novel approach to detecting active caspases: the kits take advantage of FLICA methodology, which is an affinity label. This affinity label associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amino acid sequence. Different amino acid recognition sequences are used to detect different caspases: valine-alanine-aspartic acid (VAD) for poly caspases (including caspase-1, -3, -4, -5, -6, -7, -8, and -9), aspartic acid-glutamic acid-valine-aspartic acid (DEVD) for caspase-3/7, and leucine-glutamic acid-threonine-aspartic acid (LETD) for caspase-8. A carboxyfluorescein (FAM) group is attached as a reporter.

The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining fluorescent signal is a direct measure of the amount of active caspase present at the time the inhibitor was added. The approximate excitation and emission peaks of the FLICA, propidium iodide, and Hoechst 33342 reagents are 488 nm/⁄530 nm, 535⁄ nm/6617 nm, and 350 nm/⁄461 nm, respectively. The detection reagents included within the individual Vybrant FAM Poly Caspases Assay, Caspase-3 and -7 Assay, and Caspase-8 Assay apoptosis kits are 488⁄⁄530 FAM-VAD-FMK reagent, 488/⁄530 FAM-DEVD-FMK, and 488/⁄530 FAM-LETD-FMK, respectively.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Vybrant FAM Caspase-3 and -7 Assay Kit
Tube
Caspase Assay Kit
FAM-DEVD-FMD: 388/530
PI: 535/617
Hoechst 33342: 350/461
Vybrant
Flow Cytometer
Other Label(s) or Dye(s)
25 assays
1 vial FAM-DEVD-FMK caspase-3 and -7 reagent (FLICA reagent, lyophilized solid), 1 vial of Hoechst 33342 (400 μL, 1 mM in water), 1 vial of PI (1 mL, 250 μg/mL in water), 1 vial of DMSO (0.5 mL), 1 bottle of apoptosis fixative solution (10% formaldehyde with methanol in PBS, 6 mL), and 1 bottle of 10x apoptosis wash buffer.
UV, 488
Room Temperature
Fluorescence
FAM, Hoechst 33342, Propidium Iodide
Store in refrigerator (2–8°C) and protect from light.
Produktvorschläge

Produktvorschläge

Videos
Sicherheit und Handhabung

Sicherheit und Handhabung

Produktidentifikator
  • Vybrant FLICA Caspase Apoptosis Assay Kits for flow cytometry

Signalwort
  • Warning

Gefahrenkategorie
  • Acute toxicity Category 4
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 2

Gefahrenhinweis
  • H302-Harmful if swallowed.
  • H312-Harmful in contact with skin.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
  • H351-Suspected of causing cancer.
  • H371-May cause damage to organs.

Sicherheitshinweis
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Ergänzende Informationen
  • MIXTURE LIST-Contains : Formaldehyde, methanol

Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Aktionen

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