missing translation for 'onlineSavingsMsg'
Learn More

Molecular Probes™ Vybrant™ DyeCycle™ Orange Stain

Vybrant™ DyeCycle™ Orange Stain

Brand:  Molecular Probes™ V35005

Additional Details : Weight : 0.02000kg

Product Code. 10013072

  • 6290.00 NOK / Each
In Stock
to see stock.
Add to basket

Description

Description

Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.

  • Precise—accurate cell cycle analysis in living cells

  • Safe—low cytotoxicity for cell sorting and additional live cell experiments

  • Simple, robust staining protocol

    View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

    Precise

    Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

    Low Cytotoxicity

    Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

    Simple, Robust Staining Protocol

    For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Orange Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
  • Specifications

    Specifications

    5 mM
    Fluorescent
    Stain
    532, 488
    Tube(s)
    200
    Water
    Contains 1 vial of Vybrant™ DyeCycle™ orange stain (5 mM in DMSO). Store at ≤-20°C, desiccate and protect from light.
    Flow Cytometer
    Vybrant™ DyeCycle™ Orange
    Solution
    DyeCycle™
    Room Temperature
    Nucleic Acids
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    For Research Use Only. Not for use in diagnostic procedures.