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Thermo Scientific™ Verso 1-step RT-qPCR Mix, low ROX
Descripción
The Thermo Scientific Verso 1-step RT-qPCR Low ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA.
An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.
- Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
- RT and qPCR steps performed in single tube - convenience and reduced contamination risk
- Use of the entire RT reaction as template for qPCR - improved sensitivity
- Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg
- Verso RT enzyme has a wide working temperature range (42 to 57°C) - improved success with GC-rich and other difficult templates
- Contains an inert blue dye for ease of pipetting during reaction set up
Especificaciones
Especificaciones
| Content And Storage | Contains: •1 x 50 μL Verso Enzyme Mix •1 x 250μL RT enhancer •2 x 1.25 mL 2X 1-Step qPCR Low ROX Mix Store at -20°C |
| Detection Method | Primer-probe |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | 1-step RT-qPCR |
| For Use With (Equipment) | 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P |
| Product Line | Verso |
| Product Type | 1-Step RT-qPCR Mix |
| Quantity | 200 reactions |
| Sufficient For | 200 Reactions |
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Frequently Asked Questions (FAQs)
The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.
No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.
For Research Use Only. Not for use in diagnostic procedures.
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