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  7. Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Product Code. 13505270 Shop All Thermo Scientific Products
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Sufficient For:
250 Reactions
500 Reactions
5000 Reactions
Unit Size:
1250 reactions
250 reactions
500 reactions
5000 reactions
Product Code. Sufficient For unitSize
13505270 250 Reactions 1250 reactions
13585260 500 Reactions 500 reactions
13575260 250 Reactions 250 reactions
13595260 5000 Reactions 5000 reactions
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Product Code. 13505270

Brand: Thermo Scientific™ K0993

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This item is not returnable. View return policy

Description

Includes

Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Contains:
• 10 x 1.25 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1250 x 20 μL reactions
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Line Luminaris
Product Type qPCR Master Mix
Quantity 1250 reactions
Sufficient For 250 Reactions
For Use With (Application) Gene Expression
Fidelity (vs. Taq) 2 X
No. of Reactions 1250 Reactions
Sample Type DNA, dsDNA
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For Research Use Only. Not for use in diagnostic procedures.

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