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  7. Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Product Code. p-4861499 Shop All Thermo Scientific Products
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No. of Reactions:
1250 Reactions
250 Reactions
500 Reactions
5000 Reactions
Unit Size:
1250 reactions
250 reactions
500 reactions
5000 reactions
Product Code. No. of Reactions unitSize
13505260 500 Reactions 500 reactions
13585250 250 Reactions 250 reactions
13515260 5000 Reactions 5000 reactions
13525260 1250 Reactions 1250 reactions
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Product Code. 13505260

Brand: Thermo Scientific™ K0972

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This item is not returnable. View return policy

Description

Includes

Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-ti

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
TRUSTED_SUSTAINABILITY

Specifications

Concentration 2X
Content And Storage Contains:
• 4 x 1.25 mL Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 500 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 4 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method SYBR
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Product Line Luminaris
Product Type qPCR Master Mix
Quantity 500 reactions
Sufficient For 500 Reactions
For Use With (Application) Gene Expression
Fidelity (vs. Taq) 2 X
No. of Reactions 500 Reactions
Sample Type DNA, dsDNA
Volume 7.5 mL
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For Research Use Only. Not for use in diagnostic procedures.

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