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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX
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No. of Reactions:
1250 Reactions
250 Reactions
500 Reactions
5000 Reactions
Unit Size:
1250 reactions
250 reactions
500 reactions
5000 reactions
Description
Includes
Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-ti
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Specifications
Specifications
| Concentration | 2X |
| Content And Storage | Contains: • 4 x 1.25 mL Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 500 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms. • 4 x 1.25 mL Nuclease-Free Water Store at -20°C. |
| Detection Method | SYBR |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P |
| Product Line | Luminaris |
| Product Type | qPCR Master Mix |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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