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Description
Includes
Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: ingle copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Specifications
Specifications
| Concentration | 2X |
| Content And Storage | Contains: • 4 x 12.5 mL Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms. • 2 x 30 mL Nuclease-Free Water Store at -20°C. |
| Detection Method | SYBR |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System |
| Product Line | Luminaris |
| Product Type | qPCR Master Mix |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
Product Title
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