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Cytiva TempliPhi™ Sequence Resolver Kit

Product Code. p-3973106 Shop All Cytiva Products
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No. of Reactions:
20 reactions
200 reactions
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10308259

Brand: Cytiva 28903531

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This item is not returnable. View return policy

Includes

Sample buffer, Reaction buffer and Enzyme mix

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

  • Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
  • Requires 20 minutes hands-on time
  • Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
  • Improve difficult template sequencing success with up to 820 bp Phred 20 read length
  • For use directly in cycle sequencing reactions without purification
  • Available in sizes of 20, 50 and 500 reactions

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Description illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
For Use With (Application) Amplification of difficult templates for successful DNA sequencing
Includes 1 x 900μL Sample Buffer, 1 x 900μL Reaction Buffer, 1 x 100μL Enzyme mix
No. of Reactions 200 reactions
Product Type illustra TempliPhi Sequence Resolver Kit
Test Time 18 h
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Product Identifier
  • TempliPhi Sequence Resolver Kit, 200 reactions
Signal Word
  • Warning
Hazard Category
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P405-Store locked up.
Supplemental information
  • MIXTURE LIST-Contains : glycerol
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