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Applied Biosystems™ TaqMan™ Protein Expression Assay Kit (hCSTB)

Product Code. 10045894 Shop All Applied Biosystems Products
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Product Code. 10045894 Supplier Applied Biosystems™ Supplier No. 4405465

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Description

TaqMan™ Protein Expression Assay Kit (hCSTB)

Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assay Kit (Human CSTB) contains the assay probes and a dilution buffer for the labelling of human cystatin-B protein (hCSTB) in cell lysates prepared using the Protein Expression Sample Preparation Kit. The assay probes consist of two oligonucleotides conjugated to antibodies that bind to separate epitopes of the hCSTB protein. Key features of the TaqMan™ Protein Expression Assay:
  • Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry
  • Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform
  • Allows greater sensitivity and a more quantitative readout than western blots and immunostaining
    • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage Contains Assay Probe A, Assay Probe B, and Assay Probe Dilution Buffer. The assay probes (Assay Probe A and Assay Probe B) are supplied at a 20x concentration. The Assay Probe Dilution Buffer is supplied at a 1x concentration. Store at -15 to -25°C.
    Detection Method Primer-probe
    GC-Rich PCR Performance Low
    PCR Method qPCR
    Polymerase AmpliTaq Fast DNA Polymerase
    Reaction Speed Fast
    Technique Proximity Ligation Assay
    For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
    Green Features Sustainable packaging
    Label or Dye FAM
    Passive Reference Dye ROX (Pre-mixed)
    Product Line TaqMan
    Product Type Protein Expression Assay Kit
    Purity or Quality Grade UP (Ultra Pure)
    Quantity 100 reactions
    Shipping Condition Wet Ice
    Sufficient For 100 Reactions
    Target Specificity Target-Specific
    Hot Start No
    No. of Reactions 100 reactions
    Sample Type Cells
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    For Research Use Only. Not for use in diagnostic procedures.

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