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Thermo Scientific™ Tango™ HRH3-bla U2OS DA Assay Kit

Product Code. 10277173
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Quantity:
1 Plate
Unit Size:
Each
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Product Code. 10277173

Brand: Thermo Scientific™ K1509

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Description

The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine. In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_007232
Assay Entry Cell-based beta-arrestin recruitment
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• Tango™ HRH3-bla U2OS DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• Tango™ HRH3-bla U2OS Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Detection Method Fluorescence
For Use With (Application) GPCRs
Gene Symbol HRH3
Includes HRH3-bla U2OS DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70 μg, Solution D, Tango™ HRH3-bla U2OS Assay Protocol, LiveBLAzer™FRET B/G Loading Protocol, Certificate of Analysis
Population Status Pool of Stable Cells
Product Line Tango
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry HRH3, H3
Type Division-Arrested Cells Assay Kit
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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