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Thermo Scientific™ Tango™ EDG3-bla U2OS Cells
Description
Tango™ EDG3-bla U2OS cells contain the human Endothelial Differentiation Gene 3 (EDG3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ EDG3-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of S1P. In addition, Tango™ EDG3-bla U2OS cells have been tested for assay performance under variable conditions.
Specifications
Specifications
| Accession Number | NM_005226.2 |
| Assay Entry | Cell-based beta-arrestin recruitment |
| Cell State | Dividing Cells |
| Content And Storage | Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
| Cytoplasmic Tail | Native Tail |
| Detection Method | Fluorescence |
| For Use With (Application) | FRET |
| Human Protein Class | G-Protein Coupled Receptor (GPCR) |
| Population Status | Pool of Stable Cells |
| Product Line | Tango |
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For Research Use Only. Not for use in diagnostic procedures.
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