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Thermo Scientific™ Tango™ ADORA1-bla U2OS Cells
Description
The Tango™ ADORA1-bla U2OS cells contain the human Adenosine A1 receptor (ADORA1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ ADORA1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of NECA. In addition, Tango™ ADORA1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Spécification
Spécification
| Accession Number | NM_000674.1 |
| Assay Entry | Cell-based beta-arrestin recruitment |
| Cell State | Dividing Cells |
| Content And Storage | LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ ADORA1-bla U2OS cells • Tango™ ADORA1-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ ADORA1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Cytoplasmic Tail | Tail from AVPR2 GPCR |
| Detection Method | Fluorescence |
| For Use With (Application) | GPCRs |
| Gene Symbol | ADORA1A |
| Includes | ADORA1-bla U2OS cells, ADORA1-bla U2OS Assay Protocol, Certificate of Analysis |
| Population Status | Pool of Stable Cells |
| Afficher plus |
For Research Use Only. Not for use in diagnostic procedures.
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