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  6. Thermo Scientific™ SuperSignal™ Enhanced Molecular Weight Protein Ladder

Thermo Scientific™ SuperSignal™ Enhanced Molecular Weight Protein Ladder

Product Code. p-7225572 Shop All Thermo Scientific Products
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Quantity:
250 μL
4 x 250 μL
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1000µL
250µL
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Code produit. Quantity unitSize
10373488 250 μL 250µL
17198178 4 x 250 μL 1000µL
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Code produit. 10373488 missing translation for 'mfr' Thermo Scientific™ missing translation for 'supplierNo' 84786

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Description

Ready-to-use mix of recombinant proteins (20 to 150K) with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection methods.

Thermo Scientific™ SuperSignal Molecular Weight Protein Ladders (20 to 150KDa) are a ready-to-use mix of recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed wi

The SuperSignal Molecular Weight Protein Ladders and SuperSignal Enhanced Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The molecular weight marker mix also contains a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.

Two versions of the standard are offered. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced standard is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used.

Highlights:

  • Ready-to-use – liquid format is ready to load and requires no boiling

  • Direct visualization – see marker bands with gel stains and blotting detection reagents

  • Consistent – even band intensity regardless of detection method

  • Compatible – enhanced version for compatibility with mouse monoclonal primary antibodies

  • Simple marker sizes – band molecular weights are divisible by 10K, making them easy to remember

  • Stable – store long term at -20°C, three months at 4°C or one month at ambient temperature

Additional Features:

  • The molecular masses included in these standards are 20, 30, 40, 50, 60, 80, 100 and 150kDa.
TRUSTED_SUSTAINABILITY

Spécification

Content And Storage Contents: one vial of 250 μL

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient
Detection Method User defined detection system
Number of Markers 8
Ready to Load Yes
Size Range 20 to 150 kDa
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 150, 100, 80, 60, 50, 40, 30, 20 kDa
Quantity 250 μL
Shipping Condition Wet Ice
Product Line SuperSignal
Product Type Protein Ladder
Stain Type Unstained
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.

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