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  5. Thermo Scientific™ StartingBlock™ Blocking Buffer

Thermo Scientific™ StartingBlock™ Blocking Buffer

Product Code. 10371804 Shop All Thermo Scientific Products
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Quantity:
100 mL
1 L
3 x 1 L
Chemical Name or Material:
PBS
PBST
TBS
TBST
Unit Size:
1 L
100mL
Pack of 3
7 product options available for selection
Product selection table with 7 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Chemical Name or Material Unit Size
10371804 1 L TBS 1 L
13414299 100 mL PBS 100mL
10108313 1 L PBS 1 L
13424299 100 mL TBS 100mL
30089296 3 x 1 L PBS Pack of 3
10270404 1 L PBST 1 L
10381804 1 L TBST 1 L
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 7 options available.
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Product Code. 10371804 Supplier Thermo Scientific™ Supplier No. 37542
Protein Biology Essentials
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Beskrivelse

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.

Features of StartingBlock Blocking Buffer include:

  • Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
  • Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
  • Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells
TRUSTED_SUSTAINABILITY

Tekniske data

Chemical Name or Material TBS
Concentration 1X
For Use With (Application) Western Blot
Physical Form Liquid
Product Line StartingBlock
Quantity 1 L
Formulation Proprietary protein formulation in phosphate-buffered saline, pH 7.5

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.
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