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  5. IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

IBA Lifesciences StarGate pPSG-IBA Acceptor Vectors for E. coli Expression

Product Code. 15613768 Shop All IBA Lifesciences Products
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Vector:
pPSG-IBA 105
pPSG-IBA 123
pPSG-IBA 164
pPSG-IBA 168
pPSG-IBA 23
pPSG-IBA 25
pPSG-IBA 3
pPSG-IBA 33
pPSG-IBA 5
pPSG-IBA 62
pPSG-IBA 63
pPSG-IBA 64
pPSG-IBA 65
pPSG-IBA wt1
pPSG-IBA103
pPSG-IBA143
pPSG-IBA145
pPSG-IBA162
pPSG-IBA167
pPSG-IBA35
pPSG-IBA43
pPSG-IBA45
Unit Size:
5µg
22 product options available for selection
Product selection table with 22 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Vector unitSize
15613768 pPSG-IBA45 5µg
15633758 pPSG-IBA wt1 5µg
15643758 pPSG-IBA 3 5µg
15653758 pPSG-IBA 5 5µg
15663758 pPSG-IBA 23 5µg
15673758 pPSG-IBA 25 5µg
15683758 pPSG-IBA 33 5µg
15693758 pPSG-IBA35 5µg
15603768 pPSG-IBA43 5µg
15623768 pPSG-IBA 62 5µg
15633768 pPSG-IBA 63 5µg
15643768 pPSG-IBA 64 5µg
15653768 pPSG-IBA 65 5µg
15663768 pPSG-IBA103 5µg
15673768 pPSG-IBA 105 5µg
15683768 pPSG-IBA 123 5µg
15693768 pPSG-IBA143 5µg
15603778 pPSG-IBA145 5µg
15613778 pPSG-IBA162 5µg
15623778 pPSG-IBA 164 5µg
15633778 pPSG-IBA167 5µg
15643778 pPSG-IBA 168 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 22 options available.
22 options
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Product Code. 15613768 Supplier IBA Lifesciences Supplier No. 54245001

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Description

Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.

pPSG-IGA E. coli expression vectors:

pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest.

  • As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.

    • High-level expression in E. coli by bacteriophage T7 promoter
    • High-level transcription by T7 RNA polymerase in BL21 strains
    • High-level expression of non-toxic proteins
    • Induction by IPTG
    • Suitable for in vitro transcription/translation

    StarGate System

    IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

    • Minimal extra modification of the gene due to short combinatorial sites
    • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
    • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
    • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
    • The final expression vector, the Destination Vector, is then placed into the respective host

    • Specifications

    Format Liquid
    For Use With (Equipment) StarGate™ System, E. Coli BL21(DE3)
    Inducing Agent E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3))
    pH 8
    Promoter T7
    Product Type Acceptor Vector for E. coli expression
    Storage Buffer 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
    Content And Storage 2°C to 8°C for frequent usage, -20°C for long-term storage
    Protein Tag Strep-tag for N-terminal and His Tag (6x) for C-terminal
    Concentration 250 ng/μL
    Quantity 5 μg
    Vector pPSG-IBA45
    Color Clear
    For Use With (Application) Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
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