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MP Biomedicals™ SPINeasy™ Plasmid Midiprep Kit

Product Code. 17894126 Shop All MP Biomedicals Products
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Quantity:
25 Preps
Unit Size:
25 preps
This item is not returnable. View return policy

Product Code. 17894126

Brand: MP Biomedicals™ 116539025

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This item is not returnable. View return policy

Description

Spin column technology for fast and efficient purification of both high and low copy plasmids from bacterial cultures. High-quality plasmid DNA is free of gDNA contamination following a modified alkaline lysis method.

  • SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 mL of transformed bacterial cultures.
  • Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA.
  • Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the supernatant is adsorbed on a silica membrane, washed, and eluted in a convenient process using spin column technology.
  • Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology applications, including cloning, in-vitro transcription, PCR, and restriction digestion.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at Room Temperature, Dry Environment
Isolation Technology Spin Column
Sample Type 25 to 50 mL Bacterial (E. coli) Cultures
Includes 60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
Quantity 25 Preps

Safety and Handling

compliance-icons
Product Identifier
  • SPINeasy Plasmid Midiprep Kit
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P321-Specific treatment (see on this label).
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Guanidine Hydrochloride

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.

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