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SPHK1 Recombinant Human Protein
SPHK1 Recombinant Human Protein
Brand: Invitrogen PV5214
Additional Details : Weight : 0.50000kg
Description
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signalling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapuetics for the treatment of cancer.Specifications
| NP_068807.2 | |
| Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| SPHK1 Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| SPHK1 Recombinant Human Protein | |
| SPHK and DGCK | |
| 1,040nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. SPHK1 activity determined at a final protein concentration of 0.977ng/mL | |
| GST Tag | |
| Kinase and inhibitors | |
| 95% by SDS-PAGE |
| 0.37mg/mL | |
| 71.4kDa | |
| 20μg | |
| For research use only. Not for use in diagnostic procedures | |
| SPHK1 | |
| SPHK1 was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 1μM ATP and 50μM Sphingosine kinase substrate (PV5372) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 1.6nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation | |
| SPHK1 Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Lipid kinase |
For Research Use Only. Not for use in diagnostic procedures.