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Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Protein A Kits
Description
Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well for your biomarker studies.
Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Protein A Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The SMART Digest IA Protein A Kits target purification of immunoglobulins due to the strong affinity for binding to the Fc portion of the molecule.
The need to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.
As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.
Immunocapture and Protein Digestion in a Single WellThese IA kits deliver a process that is:
- Fast
- Simple
- Highly reproducible
- Sensitive
In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.
Specifications
Specifications
| Description | Magnetic with SOLAμ SPE and collection plate |
| Capture | Protein A |
| Cleavage | C-terminal side of lysine and arginine amino acids |
| Enzyme | Trypsin |
| Format | SMART Digest Resin, Magnetic |
| For Use With (Application) | Peptide Quantitation |
| Product Line | SMART Digest |
| Type | Immunocapture + Protein Digestion combined on a single bead |
| Includes | Magnetic SMART Digest Resin, Collection Plate, SOLAμ SPE Plate |
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