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Description
pDSG-IBA vectors for MEXi mammalian expression system
- Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
- Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number
Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Marker Gene | Ampicillin |
| Promoter | CMV |
| Product Type | Vector |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Species | Mammalian |
| Protein Tag | Twin-Strep-tag |
| Cloning Method | Direct Cloning |
| Concentration | 250 ng/μL |
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Product Title
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