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Invitrogen™ RNAqueous™ Total RNA Isolation Kit
Description
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
The RNAqueous kit provides:
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
Specifications
Specifications
| Content And Storage | • 6 mL Formaldehyde Load Dye; store at -20°C • 60 mL Lysis/Binding Solution; 4°C • 60 mL Water; 4°C • 4 mL Lithium Chloride Precipitation Solution; 4°C • 40 mL Wash Solution #1; 4°C • 80 mL Wash Solution #2/3 (concentrated); 4°C • 50 Filter Cartridges; room temperature • 100 Collection Tubes; room temperature • 10 mL Elution Solution; room temperature |
| Isolation Technology | Spin Column (Glass Fiber Filter) |
| Elution Volume | 50–120 μL |
| Sample Type | Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Product Type | RNA Isolation Kit |
| Quantity | 50 Preps |
| Shipping Condition | Room Temperature |
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Safety and Handling
- RNAqueous Total RNA Isolation Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Carcinogenicity Category 1B
- Serious eye damage/eye irritation Category 2
- Germ cell mutagenicity Category 2
- Reproductive toxicity Category 1B
- Skin corrosion/irritation Category 1 B
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H360-May damage fertility or the unborn child.
- H371-May cause damage to organs.
- H412-Harmful to aquatic life with long lasting effects.
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Lithium chloride, Formamide, Formaldehyde, Methanol, 2-Mercaptoethanol
For Research Use Only. Not for use in diagnostic procedures.
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