Invitrogen™ Quant-it™ RiboGreen Reagent and RNA Assay Kit

Quant-iT RiboGreen RNA Assay Kit
Containing one of the most sensitive detection dyes for the quantitation of RNA, the Quant-iT RiboGreen RNA Assay Kit and the RediPlate 96 RiboGreen RNA Quantitation Kit offer linear fluorescence detection in the range of 1–200 ng of RNA. Both assay kits contain Quant-iT RiboGreen RNA reagent as well as buffers and RNA standards.

RediPlate 96 RiboGreen RNA Quantitation Kit
The RediPlate 96 RiboGreen RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate is pre-loaded with RiboGreen reagent, which shows bright green fluorescence upon binding to RNA or DNA. Just add buffer and samples to the microplate wells, wait 10 minutes, and read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate 96 design is ideal for either high-throughput RNA quantitation or the measurement of a small sample numbers.

The RediPlate 96 RiboGreen RNA Quantitation Kit also offers the following benefits:
• Automatable—a 10 minute incubation time with no separation steps makes the assay ideal for automated, high-throughput measurements
• Precise—the average coefficient of variation for replicate assays is ≤5% using a robotic liquid handling system
• Convenient—each plate includes pre-measured RNA standards and is ready for immediate use
• Easy to read—the assay can be performed using standard fluorescence-based microplate readers

Applications
The RediPlate 96 RiboGreen RNA Quantitation Kit is ideal for measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations.

Quant-iT RiboGreen RNA Reagent
The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA standard.