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R&D Systems™ Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Product Code. 18748357
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18748357 5μg
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Product Code. 18748357 Supplier R&D Systems™ Supplier No. 8908SH005

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4544.30 NOK valid until 2026-03-31
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity is derived from HEK293. The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity has been validated for the following applications: Bioactivity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q15465
For Use With (Application) Bioactivity
Gene ID (Entrez) 6469
Molecular Weight (g/mol) Observed M.W.: 18-24kDa, reducing conditions, Theoretical M.W.: 20kDa
Quantity 5μg
Source Human embryonic kidney cell,HEK293-derived human Sonic Hedgehog/Shh protein Cys24-Gly197
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
Common Name Sonic Hedgehog/Shh
Gene Symbol SHH
Biological Activity Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 6-36ng/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Human embryonic kidney cell, HEK293-derived Cys24-Gly197
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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