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R&D Systems™ Recombinant Human KGF/FGF-7 Protein, Animal-Free
Description
Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 6.00-60.0 ng/mL. The specific activity of Animal-FreeÖ Recombinant Human KGF/FGF-7 is >8.16 x 105 units/mg, which is calibrated against the human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).
Specifications
Specifications
| Accession Number | P21781.1 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose. |
| Gene ID (Entrez) | 2252 |
| Molecular Weight (g/mol) | MolecularWeight-observed: 20 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa |
| Quantity | 50 μg |
| Source | E. coli-derived human KGF/FGF-7 protein Cys32-Thr194, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | FGF7, FGF-7, fibroblast growth factor 7HBGF-7, HBGF7, HBGF-7, Heparin-binding growth factor 7, keratinocyte growth factor, KGFfibroblast growth factor 7 (keratinocyte growth factor) |
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